山羊Lcn 5的表达特点及其在繁殖器官中定位

doi:10.11843/j.issn.0366-6964.2015.05.005

Abstract

Abstract:

This study was conducted to investigate the expression characteristics of Lcn 5 mRNA and its protein localization in testes，epididymis in 5-month-old bucks and spermatozoa in adult bucks.Relative expressions of Lcn 5 mRNA were detected by real-time fluorescent quantitative PCR in 18 different tissues and epididymis at different ages.The expression of Lcn 5 protein was detected by Western blotting.The localization of Lcn 5 protein in testis and epididymidis were examined by immunohistochemistry，and in spermatozoa by immunofluorescence assay.The results of qRT-PCR showed that Lcn 5 mRNA was expressed in all tissues detected，which had higher levels in reproductive tissues than in other tissues.The order of expression levels in reproductive tissues：caput>cauda>corpus>testis.The expression of Lcn 5 mRNA in epididymis was increased with the increase of age before 5 months old，which was the highest at age of 5 months and was decreased subsequently.The results of Western blotting analysis showed order of the protein of Lcn 5 caput >cauda>corpus> testis，which verified the results of qRT-PCR.Immunohistochemistry results showed that there were strong positive signal in columnar epithelium of epididymis，and weak positive signal in testis.Lcn 5 protein was located on the sperm acrosome surface.Lcn 5 mRNA was highly expressed in epididymis，while was time-special and region-special expression.Lcn 5 was coated on sperm acrosome surface，which maybe play an important role in sperm maturation and capacitation process.Therefore，the functions need further research.

CLC Number:

S827.2

REN You-she，GUO Li-na，ZHANG Chun-xiang，ZHANG Guo-lin，XIA Long-gang，QIAO Li-ying，JIN Li，LIU Wen-zhong. Expression Characteristics of Lcn 5 and Its Localization in Reproduction Organ of Bucks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(5): 711-718.

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Expression Characteristics of Lcn 5 and Its Localization in Reproduction Organ of Bucks

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